Efficacy of Adipose Tissue Derived Stem Cells for the Treatment of Diabetic Foot Ulcers

Conditions studied

Diabetic Foot Ulcer, Diabetes Mellitus, Diabetic Foot, Diabetic Foot Infection, Diabetic Foot Ulcer Neuropathic, Foot Ulcer Due to Type 1 Diabetes Mellitus, Foot Ulcer Due to Type 2 Diabetes Mellitus, Foot Ulcer, Diabetes Complications, Chronic Diabetic Ulcer of Left Foot, Chronic Diabetic Foot Ulcer of Right Foot

About this study

The aim of this study is to evaluate the therapeutic efficacy of uncultured adipose derived stromal vascular fraction (SVF) and cultured adipose derived stem cells (ASCs) both supplemented with platelet rich plasma (PRP) to treat chronic diabetic foot ulcers. It will increase the pragmatic potential of both types of cells as PRP is rich in survival and chemotactic factors. Moreover, the autologous nature of the proposed study will ensure safety of its use in diabetic patients and will unveil the more effective therapeutic option for treatment of foot ulcer wounds.

Interventions

• Other: Control; Standard-of-care management — Patients will undergo conventional wound care management.
• Biological: Only PRP injection — Only PRP injected / cm2 intradermally at the border zone \& inside of wound surface bed.
• Biological: PRP + SVF injection — Uncultured cells (SVF) along PRP were mixed, transferred to 1 cc syringes and injected / cm2 intradermally at the border zone \& inside of wound surface bed.
• Biological: PRP + ASCs injection — Cultured ASCs along PRP were mixed, transferred to 1 cc syringes and injected / cm2 intradermally at the border zone \& inside of wound surface bed.

Primary outcomes

• Interview and visual inspection of ulcers recovery rate (3 months)

Eligibility information

Study locations

• Stem Cell Laboratory, Jinnah Burn and Reconstructive Surgery Center (JB&RSC), Lahore, Punjab Province 54550 Pakistan