"Studying The Role of Key Epigenetic Mediators in Breast Cancer Patients"

Conditions studied

Breast Cancer

About this study

The role of epigenetic regulators and their dysregulation in cancer have gained much attention recently, as they influence the gene expression of many oncogenes and tumor suppressor genes. Non-coding RNAs (ncRNAs) including (lncRNAs and miRNAs) and chromatin remodeling machinery proteins are key epigenetic regulators playing a key role in carcinogenesis. In addition, they can serve as diagnostic and prognostic markers in many cancer types including breast cancer. Therefore, studying these molecular markers will help in the diagnosis and prognosis as well as in the better understanding of this devastating disease.To the best of our knowledge, This is the first research work measuring the expression level of UPK1A-AS1 and/or UNC5B-AS1 in breast cancer clinical samples. These data could provide a promising approach in introducing a novel markers that help in the diagnosis and prognosis of breast cancer and provide a potential targets for gene therapy.

Primary outcomes

• Elucidate the role of our candidate lncRNAs and their downstream targets obtained from breast cancer patients' liquid biopsy and/or tissue samples. (around 12 months)
• Compare the expression level of lncRNA UPK1A- AS1 and/or UNC5B-AS1 and their target miRNAs in serum samples (liquid biopsy) and/or tissue samples from breast cancer patients, using (qRT-PCR) to their expression in healthy volunteers (around 12 months)
• Compare serum and/or tissue target proteins level, by ELISA to their levels in healthy volunteers. (around 12 months)
• Correlate these ncRNAs and target protein level axis, to the clinicopathological characteristics of breast cancer (around 12 months)

Eligibility information

Study locations

• Faculty of pharmacy, Ain Shams University, Cairo, Egypt